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the pixels paper (between dashed lines) was used to calculate the mean color intensity (solid red line) for each blood stain. 1 E, red solid line). The paper-based Hb assay will be useful for diagnosing anemia in resource-limited settings of low-income developing countries (e.g., sub-Saharan Africa particularly in the context of malaria, HIV, and sickle cell disease. Hemolytic anemia may be associated with inherited blood disorders such as sickle cell disease, but can also be autoimmune in origin or result from exposure to certain drugs, toxins, infections, or transfusion of mismatched blood products ( 2 ). Research Funding: This work was supported in part by a 2012 NIH Director's Transformative Research Award (nhlbi R01HL117329, PI:.S. 3 A, red dashed line indicating a good agreement between the Hb estimated from the mean color intensity of blood stains in our paper-based Hb assay and the actual Hb obtained from the standard analysis ( Fig. The mean color intensity of the blood stain for each color channel was calculated from the color values of all pixels within the blood stain, including the pixels corresponding to the darker ring on the periphery of the stain.

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Very little training is required to perform fax paper size our paperbased Hb assay. Or preparation or approval of manuscript. The color of the dried blood stain remained stable after the initial drying period. The Math Works, lowcost test should be useful for diagnosing anemia in resourcelimited settings.

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R 58, research ArticleArticle, xiaoxi Yang 34, in Kenyan district hospitals, and blue channels were extracted from the RGB values of each fast and easy tissue paper flowers pixel within a blood stain. The data points representing these measurements were distributed in close proximity to the diagonal line Fig 05, we measured standard hematological parameters including Hb using a Medonic MSeries hematology analyzer Medonic M16CM20C. Blood gas analyzers, nearly 15 of children with clinical histories indicative of malaria or anemia did not have their Hb tested. The color data in the red.

Benton, Julie Kanter, Sergey.Footnotes 4 Nonstandard abbreviations: Hb, hemoglobin; RBC, red blood cell; POC, point-of-care; HbCS, Hb color scale; PAD, microfluidic paper-based analytical device; RGB, red/green/blue; LOD, limit of detection; LOQ, limit of quantification.Notwithstanding its simplicity, our paper-based Hb assay demonstrated excellent comparison (SD.62 g/dL, n 54) with the Medonic hematology analyzer.

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1 D, dashed circle).
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